Pdf the ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and. The smartcycler system is the only system that enables random access to help speed your assay development. When it comes to fighting crop diseases, field surveillance also has its place on the front lines of farming. You can configure the smartcycler system to suit your exact needs. Management of software engineering and systems integration organizations. Over a decade ago, cepheid set the standard for innovation in automated molecularsee this and similar jobs on linkedin. The smartcycler system software protocol run with advance to next stage feature off. Cepheid s genexpert system is the worlds most flexible platform, providing healthcare professionals with a best in class test menu for any setting from the core lab to near patient applications. Cepheid s smartcycler system allows users to easily develop and optimize their own assays. Fluorescence from fam reporter was detected at 505537 nm wavelengths. Csu surplus property is selling a cepheid smart cycler ii td realtime pcr system previously used in a university setting.
This estimate is based upon 4 cepheid software engineer salary reports provided by employees or estimated based upon statistical methods. Activate the smart cycler software on the computer. The smart cycler software ensures ease of use for the sixteen independently controlled icore. For the smart cycler, data were analyzed by using the cepheid smart cycler software version 1. View david persings profile on linkedin, the worlds largest professional community. Unit is block only does not include any software or manuals. Supplier shall warrant all smart cycler system products for 12 months from delivery to fishers customer and all accessories except the computer supplied with a product for 12 months from delivery to fishers customer. Smartcycler system is a leading realtime pcr testing platform for research hospitals, university labs and government agencies. Each of these randomaccess, realtime, modular pcr processing units can test 16 different protocols simultaneouslythermal cyclers are used to amplify segments of dna via the polymerase chain reaction. Apr 11, 2006 the cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. The ability of the portable cepheid smartcycler realtime pcr machine to detect footandmouth disease fmd virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the realtime pcr assay currently in use in the laboratory.
The application of realtime polymerase chain reaction pcr in laboratory diagnostic testing. Smartcycler primary dye excitation and emission spectra. Many of cepheid s reagents are delivered in our proprietary bead technology. Pdf detection of biological threat agents by realtime pcr. The warranty provided for the computer by the computer manufacturer will be transferred to fishers customer. As testing volumes expand, you can add more capacity to the system at any time.
The equipment we offer is mainly focused towards biotech research and development, but. Based on multiplesequence alignments, we have designed two pairs of pcr primers that efficiently amplify all variants of the shiga toxin. Polymerase chain reaction testing was not developed for hsv2 because of a. Cepheid officially launched the fullyautomated genexpert system into the clinical market with the release of xpert gbs, a test for intrapartum screening of group b streptococcus in labor and delivery, in 2005. Also used to facilitate other temperaturesensitive reactions, the devices can complete restriction enzyme digestion or rapid diagnostics. The assay has been validated using cepheid smartcycler ii software version 3.
Cepheid senior software engineer interview questions. Diagnostic testing systems no matter what your testing needs, weve got the right fit. Ars, cepheid team to make plant disease diagnosis faster. Cepheid hiring software engineering intern in san jose. Research and development efforts have shifted substantially from smart cycler development in 2000 to genexpert development in 2001. Objective to compare the smartcycler ii system cepheid, sunnyvale, calif results with those of standard cell culture, to compare the smartcycler ii system results with those of a dedicated polymerase chain reaction facility, and to establish the smartcycler ii system as a polymerase chain reaction method for detecting viral and chlamydial dna from ocular specimens. Upload files that contain software or other material protected by intellectual property laws or by rights of privacy of publicity unless you own or control the rights thereto or have received all necessary consents. Two fielddeployable realtime pcr platforms were used, the smartcycler cepheid using propriety singlereaction smart tubes and the lightcycler nano roche using 8well strip tubes. A sample was deemed positive if fluorescence exceeded the threshold. Upload files that contain viruses, corrupted files. We have developed a rapid 1h realtime fluorescencebased pcr assay with the smart cycler thermal cycler cepheid, sunnyvale, calif. Development of a novel internal positive control for taqman. Smartcycler pcr systems, cepheid smartcycler smart cycler ii. Our it staff was able to power on the smart cycler and included laptop.
Cepheid smart gbs test for group b streptococcus, run on. Intern 3 engineering 3 quality engineer 3 software engineer 2 internship 2 senior software engineer 2 project manager 2 research associate 2 software engineering 1 inventory analyst 1 intern hourly 1 senior supplier development engineer 1 senior technical writer 1. Cepheid s portable smart cycler unit, which connects to a laptop computer, uses polymerase chain reaction pcr to replicate a target pathogens dnaits unique genetic. Cepheid s smartcycler instrument is the only randomaccess, modular realtime. Development of a rapid, sensitive taqman realtime rtpcr. Evaluation of the smartcycler ii system for realtime.
Cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. The laptop connects to the cycler and recognizes it. Cepheid genexpert molecular diagnostic testing systems. The focus on quality begins at the earliest stage of product design and carries through to the end user. The optical system of the unit is centered around the icore, a solid state module which does not require warm up, maintenance, or normalization dye. Jan 01, 2003 cepheid s smart cycler instrument facilitates the speed and accuracy of the idistrep b test. Cepheid software test engineer salaries in the united. Smallpox and panorthopox virus detection by realtime 3.
Increase robustnessshorter cycling times extend the life of. The smartcycler is operated with cepheid smartcycler version 2. Cepheid launches ce mark certification smart vzvtm in vitro diagnostic test for smartcyclerr platform read this article along with other careers information, tips and advice on biospace. Cepheid genexpert iv dx molecular diagnostic system 6. In just over 2 hr, the smartchip realtime pcr cycler can process 5,184 reactions in parallel from a single chip. Post a free request for proposal for a cepheid smart cycler pcr system w statspin m92022 centrifuge and get quotes from our gold service dealers. Cepheid smart gbs test for group b streptococcus, run on the. Commercial off the shelf cots smartcycler gene expression analysis researchers can receive results within 24 hours of experiment run onorbit data can be used to guide the experiment in realtime allow researcher to change details timeline, etc. Data acquisition and analysis were performed using cepheid smart cycler software version 1. Find and purchase cepheid smartcycler products at invitrogen life science technologies. The cycle threshold ct values for each reaction were calculated automatically by the cepheid smart cycler software version 1. Cepheid launches ce mark certification smart vzvtm in vitro. Each thermal cycle is equipped with highperformance.
Cepheid hiring product manager in sunnyvale, california. Fda grants 510k clearance, clia waiver to cepheid flu. A fourplex, drieddown bead cartridge containing pcr reagents specific for the pxo1 and pxo2 plasmids as well. Govdeals is the place to bid on government surplus and unclaimed property including heavy equipment, cars, trucks, buses, airplanes, and so much more. Cepheid launches ce mark certification smart cmvtm in. I spoke with an internal recruiter on the phone, was sent a couple of questionnaires to fill out regarding my skill level, and was set up with a phone interview with a couple of members on the software engineering team. S authorized distributor for smart cycler products life science research, environmental, pharmaceutical, ivf quality control and cosmetics contact phone. The smart cycler starter system is delivered with all the equipment and software needed to obtain faster assay results. Individual ownership is the basis of the cepheid quality system. Type ii cepheids are variable stars which pulsate with periods typically between 1 and 50 days.
Along with ars lab associates reid frederick and vern damsteegt, schaad is collaborating with cepheid under a cooperative research and development agreement crada with ars. Evaluation of the smartcycler ii system for realtime detection of. Thermal cycling for the smart cycler instrument was performed as follows. Used cepheid smart cycler pcr system w statspin m92022. Sr engineering program manager software cepheid sunnyvale, ca, us. Cepheid smart cycler ii td realtime pcr system govdeals. For the smart cycler, data analysis was performed with the cepheid smart cycler software ver.
Nov 29, 2001 ars, cepheid team to make plant disease diagnosis faster, easier and more accurate by jan suszkiw november 29, 2001. Cepheid offers a genexpert system for testing in the areas of healthcareassociated infections, critical infectious disease, sexual health, virology, oncology, and genetics. The smartcycler system is highly robust and compact, with no moving. System processing for sale wireless technology for sale. We currently use the instrument for our prohibited material multiplex assay. Objectiveto compare the smartcycler ii system cepheid, sunnyvale, calif. S authorized distributor for smart cycler products life science research, environmental, pharmaceutical, ivf quality control and cosmetics contact.
The cepheid smart cycler ii system is an integrated dnarna amplification and detection instrument based on the microprocessorcontrolled icore intelligent coolingheating optical reaction module. Evaluation of cepheid smartcycler and euroclone dual easy ct. Smartmix and omnimix are available for convenience which contain all the reagents to perform a pcr reaction, except the primers and probes, in a. The smart cycler software ensures ease of use for the sixteen independently controlled icore modules found on the smart cycler processing block. Surplus property division only tests the units by plugging them up to see if they power on. I agree that cepheid shall have no obligation to pay me or otherwise compensate me in any way for receiving and evaluating the submission, and that any decision by cepheid to partner, purchase or license the submission or any intellectual property associated with the submission shall be at cepheids sole discretion. Find out why cepheid s solutions are a top choice for fast, accurate, and easy diagnostic testing. Cepheid smartcycler system software license agreement license grant. The smartcyclerr system is a leading realtime pcr testing platform for hospitals, university research labs and government agencies.
Cepheid smartcycler system software license agreement. Cepheid hiring sr engineering program manager software. Comparative evaluation of the diagnostic performance of. Find out why cepheid s solutions are a top choice for fast, accurate, and. For the smart cycler instrument, data acquisition and analysis were done by using cepheid smart cycler software version 1. Salary information comes from 4 data points collected directly from employees, users, and past and present job advertisements on indeed in the past 36 months. Designated portions of cepheid s definitive proxy statement for its 2003 annual meeting of shareholders are incorporated by reference into part iii hereof. Thermal cycler supplier agreement this agreement, effective april 15, 2000, is made by and between pe biosystems, a division of pe corporation, a corporation of the state of delaware, having an office at 850 lincoln centre drive, foster city, california 94404 pe corp, and cepheid, a corporation of the state of california, having an office at 1190 borregas avenue, sunnyvale, california. The test can provide a highly accurate result in as little as 30 minutes, during the critical period when detection of gbs may potentially reduce the number of infections and deaths to newborns caused by the bacterium. New york genomeweb the us food and drug administration has granted 510k clearance and clia waiver to an assay for influenza and respiratory syncytial virus manufactured by cepheid, the firm announced today. Leads the planning and implementation of multiple software engineering development programs. Cepheid software engineering interview questions glassdoor. It also provides a smartcycler system, which integrates dna amplification and detection to allow analysis of a sample.
Thermal cycler supplier agreement pe biosystems and cepheid. By automating the entire amplification and detection process, the smartcycler system can deliver highly accurate and consistent test results from prepared biological samples in 3040 minutes. Thresholds were set 10 standard deviations above the background. Cepheid enabling access to molecular diagnostic testing. Analysis settings consisted of a primary curve with a manual threshold of 10, background subtraction turned on, and boxcar average set to 5 cycles.
Subject to the terms and conditions herein, cepheid grants customer a nonexclusive, nontransferable license the license to use one 1 copy of the cepheid smartcycler system software program the software on the computer provided with the smart. Computer is windows 2000 with cepheid smart cycler version 2. Find out why cepheids solutions are a top choice for fast, accurate, and easy diagnostic testing. Dec 11, 2006 cepheid smart gbs test for group b streptococcus, run on the smartcyclerr system, receives 510k fda clearance read this article along with other careers information, tips and advice on biospace. The application of realtime polymerase chain reaction pcr in laboratory diagnostic testing for. Gene expression analysis can be performed using dye or probebased methods, depending on your research needs. Distribution agreement cepheid and fisher scientific co. Cepheid the cepheid smartcycler ii is a modular real time pcr system. Since then, 20 fdacleared tests and 27 ceivd tests have been introduced from healthcare associated infections to womens health, sexual health, and virology.
Evaluation of the portable cepheid smartcycler realtime pcr. Cepheid smartcycler ii thermal cycler automated realtime pcr system 10268. Software quality specialists primary responsibility is software validation and representing software quality in software development. Detection of biological threat agents by realtime pcr. Rtpcr reactions were performed using the cepheid smartcycler v. The theoretical tm can be calculated using software available for free on the world wide web refer to. Smartchip realtime pcr system technical specifications.
A threshold cycle ct was determined for each sample using the exponential growth phase and the baseline signal from fluorescence versus cyclenumber plots. Smart cycler settings consisted of a primary curve analysis with a manual threshold setting of 10, background subtraction turned on, boxcar average set to 5 cycles, background minimum cycle set to 5, and background maximum cycle set to 45. For the lightcycler instrument, thermal cycling was performed as follows. Cepheid smart cycler system authorized thermal cycler 1 serial. David persing software development engineer at adobe. Background fluorescence was automatically determined by the analysis software using raw data from cycles 3 to 10. Cepheid s scaleable smartcyclerr and genexpertr technologies are making it easier and cost effective for institutions of any size to reap the benefits of molecular diagnostics.
Cepheid is committed to providing high quality products and services that consistently meet or exceed customer expectations. Manage diverse teams responsible for new hardware and software product development as well as sustaining activities. At cepheid, software engineering and software quality work in crossfunctional agile scrum teams developing cloud services for disease surveillance and systems administration in the diagnostics area. Cepheid smart cycler ii rtpcr model sc2 with 16 well block, including computer with software. The smartcycler is a realtime pcr instrument with 16, 34, 48, 64, 80 or 96. Cepheid s menu of fdacleared tests and platform flexibility means that faster answers are available in every setting. Cepheid smartcycler ii thermal cycler automated realtime pcr. No other system is designed for complete portability in the field. Pdf evaluation of the portable cepheid smartcycler realtime. Rapid detection of shiga toxinproducing bacteria in feces. Cepheids smartcycler thermal cycler is a licensed realtime thermal cycler under appleras european patent no.
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